Crystallization intracellular

Figure 3. Intracellular freezing of 8-cell mouse embryos cooled at 20 °C/min in 2 M DMSO. The black "flashing" occurring in cells at -31 °C to -46 °C is characteristic of intracellular ice formation, and is caused by the scattering of light by many small highly branched ice crystals. (Modified from Rail et al., 1983.)...

FIGURE 56-1. Synovial fluid containing extracellular and intracellular monosodium urate crystals. (From Reginato AJ. Gout and other crystal arthropathies. In Braunwald E,... 

In rhodopsin, EPR studies have demonstrated a clear helical periodicity in most of intracellular loop-3, except for a couple of residues in the middle (indicated in Figure 2.5). This would suggest that TM-V and TM-VI extend way into the cytosol and that only a very short loop connects these two helical extensions. However, in the three-dimensional crystals, most of intracellular loop-3 is a rather unstructured loop. Thus, in this case, it is likely that the EPR studies tell us something about the solution structure of the receptor, which may not be clear in the x-ray structure. 

The definitive diagnosis is accomplished by aspiration of synovial fluid from the affected joint and identification of intracellular crystals of monosodium urate monohydrate in synovial fluid leukocytes. 

Storey R, Thomson WW. An x-ray microanalysis study of the salt glands and intracellular crystals of Tamarix. Ann Bot 1994 73 307-313. 

Ferritin is a globular protein complex consisting of 24 protein subunits and is the main intracellular iron storage protein in both prokaryotes and eukaryotes. Ferritin is used for immunolabeling at the electron microscope level because of its distinctive shape of the crystals and their electron density. 

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