Cryptochrome isolation

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Because of the phylogenetic relation between cryptochromes and photolyases, it appears likely that cryptochromes also possess an electron transfer as primary photoresponse. In situ and after isolation cryptochromes exist in a reduced form, because their absorption spectra are semiquinone-like. The action spectrum for hypocotyl inhibition, a Cryl-mediated response, resembles, however, the absorption spectrum of the oxidized form. This observation could indicate that the active form of Cryl exists in situ in the oxidized state, while the inactive form remains reduced. 

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