Cryoprotection composition

If the liposome preparations are intended to be stored for longer time periods, they may be frozen or lyophilized, provided that they are prepared in a phosphate buffer that contains a cryoprotectant. We use an iso-osmolar phosphate-mannitol buffer of the following composition 20 mM phosphate buffer (0.53 g/L KH PO plus 2.87 g/L Na2HP0 2Hp) plus 230 mM mannitol (42.0 g/L mannitol), (PB-Man)... 

Processes for adenovirus purification typically end with concentration, formulation, and sterile filtration operations [40, 80,106]. Concentration and formulation are usually carried out in ultra-filtration units equipped with 100-300-kDA membranes [40,106]. The exact composition of the formulation buffer will depend on the intended application, mode of administration (injectable, aerosol), and required short-term and shelf stability [104,123]. A typical liquid formulation may include an aqueous buffer supplemented with cryoprotectants (e.g., sucrose) and stabilizers such as the nonionic-surfactant polysorbate-80, the chelating agent EDTA, and the oxidation inhibitors ethanol and histidine [123]. Filtration under sterile conditions is typically performed with 0.22-pm membranes [103,106]. 

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