Cryomicrotomy for TEM and SPM

The following hints and tips are from a polymer microscopist [130] Use a diamond knife with an angle of 35° for polymer materials section thickness of about 90 nm is best to start, with change to lower temperature if there is severe compression and reduction of thickness to 60-70nm once sections are uniform slow cutting speed, below 1 mm/s, is preferred for polymer specimens. 

Dry sections are difficult to pick up without damage. Ionizing antistatic devices are used to 

A polycarbonate/styrene-co-acrylonitrile blend was examined as a representative of blends 

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