Crosslinking agents sulfhydryl reactive

Although acryloyl crosslinking agents have not been common, the reactive group has found use in the design of the sulfhydryl-reactive fluorescent probe, 6-acryloyl-2-dimethylaminonaph-thalene (acrylodan Molecular Probes) (Epps et al., 1992 Yem et al., 1992). 

Figure 4.17 DPDPB is a sulfhydryl-reactive crosslinker that forms disulfide bonds with thiol-containing molecules. The conjugates may be disrupted using a disulfide reducing agent such as DTT.

Figure 4.35 Benzyl halides and halomustards can be used as crosslinking agents reactive toward sulfhydryl groups.

Succinimidyl-4-(p-maleimidophenyl)butyrate (SMPB, Chapter 5, Section 1.6), is a heterobifunctional crosslinking agent that has an amine-reactive NHS ester on one end and a sulfhydryl-reactive maleimide group on the other. Conjugates formed using SMPB are linked by stable amide and thioether bonds. 

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