Cross-linking reagent, cleavable

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Smith, R.J., Capaldi, R.A., Muchmone, D., and Dahlquist, F. (1978) Cross-linking of ubiquinone cytoch-nome c reductase (complex III) with periodate-cleavable bifunctional reagents. Biochemistry 17, 3719-3723. 

Figure 193 The trifunctional reagent sulfo-SBED reacts with amine-containing molecules via its NHS ester side chain. Subsequent exposure to UV light can cause cross-link formation with a second interacting molecule. The biotin portion provides purification or labeling capability using avidin or streptavidin reagents. The disulfide bond on the NHS ester arm provides cleavability using disulfide reductants.

Control experiments showed that all the crosslinks were cleavable. No crosslinks were formed if the reagent was omitted, if photolysis was omitted, or if the reagent was first inactivated by treatment with ammonium acetate. These experiments proved that neither monofunctional cross-linking, nor disulfide interchange to give crosslinking in the dark occurred. If the membranes were solubilized in SDS before irradiation no cross-... 

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