Covalent compounds ethylene formation

A variety of compounds containing an olefinic bond, such as ethylene, allylisopropylacetamide (AlA), and secobaibital, can form covalent adducts on the nitrogen of the porphyrin group of the prosthetic heme leading to inactivation [382-385], Secobaibital has been shown to completely inactivate CYP2B1 with only partial loss of the heme chromophore [384, 386, 387], Isolation of the modified CYP2B1 protein and the A -alkylated porphyrins indicates that the reactive compound partitions between protein modifiea-tion, A -alkylation of the heme, and formation of an epoxide metabolite in the ratio of 0,2 0.8 59, respectively [387] (Fig, 5.17), The formation of a heme adduct in the active site of CYP2B1 was confirmed spectrally based on its typical absorption maximum at 445 nm, a characteristic fea-... 

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