Corneal perforation seal

Table II. Corneal Perforation Seal with MAP Plus Hypan...

Miki et al. (2000) used a photopolymerized liquid applied material for sealing corneal perforations. The novel photocrosslinkable methacrylated hyaluronic... 

Cyanoacrylate has been used for sealing corneal perforations, but it is not smooth or malleable, and it exhibits a hard tissue-scratching surface and requires the patient to use a soft contact lens for comfort. Another surgical limitation when using cyanoacrylate is the immediate polymerization on a wet or moist corneal surface, and that property reduces the time allocated for and increases the difficulty in applying the glue to a precise location in a well-controlled manner. 

The sealing of corneal perforations using whole enucleated bovine eyes required the same attention to tissue preparation. The epithelial layer in the bond area was removed. After the puncture of the cornea, the stroma was rinsed and blotted to remove potentially interfering aqueous humor components. The Hy-pan hydrogel material, which is 80-90% water, was routinely presoaked in PBS. Maximum bond strengths using 53 /ig of MAP per cm2 patch were obtained in 10 min after application. 

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