Copolypeptides hydrogels

Pochan D.J., Pakstis L., Ozbas B., Nowak A.P., and Deming T.J. SANS and cryo-TEM study of self-assembled diblock copolypeptide hydrogels with rich nano through microscale morphology. Macromolecules, 35, 5358, 2002. 

Such hydrogels have been suggested to be suitable for biotechnological applications (DNA delivery vehicles, cell encapsulation) [28]. Recently, amphiphilic dibock copolypeptide hydrogels of KigoL2o were used in an in vivo study where the hydrogels were injected into the mouse forebrain. Evaluation of samples displayed substantial tissue integration with little or no detectable toxicity in the central nervous system [148]. 

Yang CY, Song BB, Ao Y et al (2009) Biocompatibility of amphiphilic diblock copolypeptide hydrogels in the central nervous system. Biomaterials 30 2881-2898... 

Fig. 8 Time-dependent migration of cells into block copolypeptide hydrogel DCH) deposits in vivo, (a-d) Light-microscopic images of 3% KigoL o 1 ( ) 2 (b), 4 (c) and 8 (d) weeks after injection of 2 pL into the striatum in tissue sections stained with cresyl violet. Essentially, no cells are present in the deposits after 1 week in vivo (a). After 2 weeks in vivo (b), a number of cells have migrated into, and are scattered throughout the deposits. After 4 (c) and 8 weeks (d), the deposits are densely packed with cells. Arrowheads indicate the borders of deposit and host tissue scale bars. 25 mm). Adapted from [121]...

Ohkawa, K., Shoumura, K., Yamada, M., Nishida, A., Shirai, H., and Yamamoto, H., Photorespon-sive peptide and polypeptide systems, 14a biodegradation of photocrosshnkable copolypeptide hydrogels containing /-ornithine and 8-7-coumaryloxyacetyl-/-omithine residues, Macromol. Bio-sci, 1,149, 2001. 

Breedveld V., Nowak A.P., Sato J., Deming T.J., and Pine D.J. Rheology of block copolypeptide solutions Hydrogels with tunable properties. Macromolecules, 37, 3943, 2004. 

Nowak AP, Breedveld V, Pakstis L et al (2002) Rapidly recovering hydrogel scaffolds from self-assembling diblock copolypeptide amphiphiles. Nature 417 424-428... 

Deming et al. [53-55] also described the formation of hydrogels from di-and triblock copolypeptides based on PLLeuio-4o and PLLysgo-sso (hydrobromide) gelation occurred at a polymer concentration as low as 0.25 wt %. It is thought that the scaffold of the hydrogel is built of twisted fibril assemblies with a core of closely packed poly(L-leucine) a-helices and a corona of cationic poly(L-lysine) chains. 

Breedveld V, Nowak AP, Sato J, Deming TJ, Pine DJ (2004) Rheology of block copolypeptide solutions hydrogels with tunable properties. Macromolecules 37 3943-3953... 

A.P. Nowak, V. Breedveld, L. Pakstis, B. Ozbas, D.J. Pine, D. Pochan, et ai. Rapidly recovering hydrogel scaffolds from seh-assembhng diblock copolypeptide amphiphiles. Nature, 417,424-8,2002. 

Nowak, A.P. et al. Rapidlyrecovering hydrogel scaffolds from self-assembHng diblock copolypeptide amphiphiles. Nature, 2002,417 424-8. 

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