Coomassie Brilliant Blue R250 and

05 g of dye are dissolved in 30 ml methanol or non-denatur-ated ethanol or isopropanol. 10 ml of glacial acetic acid are added and the solution is filled up to 100 ml with ddH20. If the color does not change remarkably, the solution can be 

B destaining solution 10% acetic acid (v/v), 30% methanol or non-denaturated ethanol or isopropanol (v/v) in deionized water 

Both dyes, especially R250, are used mostly in staining gels and should be preferred. A second staining with Coomassie is recommended after silver staining. 

If Coomassie R250 (R - reddish, more sensitive than G250) is used, a TCA fixation should be avoided. If the gel was fixed with 

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Amido black is a commonly used stain, but it is not very sensitive. It is often used to visualize concentrated proteins or components that are readily accessible to dyes such as proteins that have been transferred from a gel to nitrocellulose paper. Two of the more sensitive and more frequently used stains are Coomassie Brilliant Blue (R250 and G250) and silver stains. Because these stains interact differently with a variety of protein molecules, optimization of the fixative and staining solutions is necessary. The Coomassie stains are approximately five times more sensitive than amido black and are appropriate for both agarose and polyacrylamide gels. The silver stain is approximately 100 times more sensitive than Coomassie and is typically used for polyacrylamide gels. 

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