Controller, with complementary linear

Control experiments with non complementary were performed to test the selectivity of the biosensor. With this approach, a sequence of the HBV could be quantified over the range from 3.53 xlO" to 1.08 xlO M, with a linear correlation of r = 0.9963 and a deteetion limit of 1.00 xlO M. 

The kinetics of these pyrolysis reactions were followed by several complementary methods under conditions as close to the product studies as possible. The most frequently-used ampule technique14 17) with gc analysis of 5 and the scavenger technique, with chloranil or Koelsch radical as scavenger 18), for very labile compounds 5 were complemented by the DSC method, in which the heat flow under conditions of linear temperature increase is analysed. It proved to be a particularly convenient and reliable technique 18- 21). Rates were followed over a temperature span of at least 40 °C with temperature control of 0.1-0.2 °C. All rate data and activation parameters were subjected to a thorough statistical analysis including statistical weights of errors. The maximum statistical errors in k were 3%, in AH 1 kcal mol-1 in AS 513 e.u. and in AG (at the temperature of measurement) g0.5 kcal mol-1. 

RNA polymerase binding sites. The vector is first linearized with a restriction enzyme such that transcription produces run-off transcripts, that is, transcripts derived from the insert sequence alone. The restriction enzyme is chosen such that transcription yields an RNA probe that is complementary (anti-sense) to the target mRNA. The probe transcribed from the opposite strand (sense probe) can be used as a negative control. The length of the probe is important. Probes of up to 1.5 kb are optimal. Longer probes penetrate the tissue less efficiently but can be partially degraded by alkaline hydrolysis to a more suitable size (2). 

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