Continuous Heat Sterilization of Culture Media

If the flow of the medium in the holding section were an ideal plug flow, the degree of sterilization could be estimated from the average residence time in the holding section by Equation 10.6  

A medium is to be continuously sterilized at a flow rate of 2 in a ster- 

Calculate the required length of the holding section of the sterilizer 

Microorganisms in liquids and gases can be removed by microfiltration hence, air supplied to aerobic fermenters can be sterilized in this way. Membrane filters are often used for the sterihzation of liquids, such as culture media for fermentation (especially for tissue culture), and also for the removal of microorganisms from various fermentation products, the heating of which should be avoided. 

Deviation from the ideal plug flow can be described by the dispersion model, which uses the axial eddy diffusivity f Dz (m2s-1) as an indicator of the degree of mixing in the flow direction. If a flow in a tube is plug flow, the axial dispersion is zero. On the other hand, if the fluid in a tube is perfectly mixed, the axial 

The average medium velocity in the holding section v — 2/(71 x 0.0752) = 113mh-1 

---