Consensus binding site

In Family A GPCRs we have defined 28 Themes so far. These represent most of the combinations found in drug molecules and these Themes populate the consensus binding site as illustrated in Fig. 4. The existence of a Theme, or not, is then defined by a search logic which surveys the amino acids at the particular positions. In the examples of the first 5 Themes below. 

Iron-sulfur clusters are important co-factors in electron-transfer. Type I reaction centres contain [4Fe-4S] clusters as final electron acceptors mediating ET to soluble electron carriers like ferredoxin or flavodoxin (reviewed in references 188, 224, 314, 315) In PS I three clusters (F FA and FB) have been clearly identified and spectroscopically characterized. The PsaA and PsaB subunits carry most of the ET cofactors in PS I.18178-316 Each of them provides two Cys ligands to the binding site of the interpolypeptide [4Fe-4S] cluster Fx. This binding site is identical on both core PS I subunits.317 Both [4Fe-4S] clusters FA and Fb are bound to the PS I stromal subunit PsaC. It contains two identical [4Fe-4S] consensus binding sites CxxCxxCxxxCP (C = cysteine, P = proline). 

Verkhivker GM, Bouzida D, Gehlhaar DK, Rejto PA, Freer ST, Rose PM (2002) Monte Carlo simulation of the peptide recognition at the consensus binding site of the constant fragment of the human immunoglobulin G the energy landscape analysis of a hot spot at the intermolecular interface, Proteins, 48 539-557... 

Wu, K., Wolgemuth, D.J. 1993. Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron. J. Cell Biochem. 52, 449 162. 

FIGURE I Schematic alignment of the deduced amino acid sequences of nitric oxide synthases (NOSs) and the cytochrome P-450 reductase. Depicted are consensus binding sites for heme, L-arginine, calmodulin (CaM), flavin mononucleotide (FMN), flavin-adenine dinucleotide (FAD), and NADPH. An NH2-terminal myristoylation site (myr) is present only in the endothelial constitutive NOS (ecNOS). n. Neuronal i, inducible. 

Wright, W. E, Binder, M, and Funk, W (1991) Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site Mot Cell Biol 11,4104-4110... 

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