Trypsin-dextran conjugate

Figure 2. Autolysis of trypsin (0) and trypsin-dextran conjugate (O) at pH 8.1...

Figure 4. Inactivation of trypsin (0) and trypsin-dextran conjugate (O) at 37°C and pH 8.1 in 8M urea and 5mM 2-mercaptoethanol. The broken line shows the rate of inactivation of trypsin-dextran conjugate after dextranase treatment.

Inhibition of Native Trypsin and Trypsin-Dextran Conjugate by Protease Inhibitors41... 

Figure 6. Sepharose 4B chromatography of trypsin-dextran conjugate (A). The sample contained approximately 145 mg dextran and 14 mg of trypsin. Chromatography of a mixture containing corresponding amounts of dextran and trypsin...

Takakura Y et al (1989) Control of pharmaceutical properties of soybean trypsin inhibitor by conjugation with dextran. II Biopharmaceutical and pharmacological properties. J Pharm Sci 78 219-222... 

In addition to showing improved stability in the presence of protein denaturants, we have also found dextran-conjugated enzymes to show improved activity in the presence of such agents. For example, in the absence of calcium, native trypsin is inactive in 8M urea under the same conditions the modified enzyme displays 50% of the activity measured in the absence of urea (11). 

Many enzymes are known to bind to glass, especially when in dilute solution. Common examples of enzymes that demonstrate this phenomenon are e-amylase (12), trypsin (18), ribonuclease (19) and catalase (20). Conjugation of these enzymes with dextran... 

Dextran cyclic imidocarbonate has been used to prepare soluble conjugates of a-amylase from Bacillus amyloliquefaciens and of bovine liver catalase for use in experimental enzyme therapy. The results suggest that by coupling the enzymes with a poorly antigenic macromolecule (dextran) anaphylactic reaction in animals pre-immunized with dextran may be suppressed or eliminated. Trypsin has been immobilized by reaction with dextran cyclic imidocarbonate. 

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