Conformational distributions spectral simulators

Fig. 21 (a) Distribution of single-molecule energy transfer rates of 1 obtained at 1.4 K. The open bars represent the rates determined from donor (PDI) excitation spectra, while the filled bars are calculated from the spectral overlaps according to Forster theory. The solid lines show a simulated rate distribution solely originating from different molecular conformations. This distribution has been scaled to fit both histograms, (b) Experimentally determined energy transfer rates plotted vs. the calculated spectral overlaps J. The solid line has been calculated by Forster theory. The dashed line represents a linear fit to the data... 

AS/NZ 4399 recommends that the polychromatic illumination should conform to the requirements of a solar simulator, i.e., in UV spectral distribution. However, as revealed by Fig. 14, this would only be relevant to quantifying the emission spectrum or whiteness of the sample. The source spectrum does not impose on the UV absorption measurement. 

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