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Conceptual basis and instrumentation

The fluctuations SI(t) of the fluorescence around the mean value f , defined as [Pg.364]

The FCS autocorrelation functions4 contain two types of information (Thompson, 1991)  [Pg.365]

4) In several papers, only the time-dependent part of the autocorrelation function is considered, and the definition is then [Pg.365]

The decay rate represents the average duration of the fluctuation signal. [Pg.366]

Two-photon FCS has been successfully developed it offers the advantage of 3-D resolution (see Section 11.2.1.2) (Chen et al., 2001). [Pg.366]

G(0) can yield information on molecular weights (e.g. labeled proteins and nucleic acids) and molecular aggregation. In these applications, the laser beam is focused through a microscope objective to a small spot on the specimen the latter is laterally translated through the beam by a computer-controlled microstepping stage, at a speed higher than the rate of translational diffusion of the species under study. This technique is called scannir -FCS. [Pg.366]


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