Oxyhemoglobin concentration

Analyses were made for oxygen content in a Van Slyke-Neill constant-volume blood gas apparatus. The transfer pipet was calibrated for content and retention on the walls of whole blood or concentrated oxyhemoglobin solution corresponding to conditions of use the gas pipet was also calibrated for volume. Correction was made for dissolved oxygen on the assumption that the quantity dissolved is proportional to the water present in the solution. 

Figure 3 Manufacturing scheme for liposome-encapsulated hemoglobin (LEH). Lipid phase is mixed with hemoglobin and the mixture is homogenized in an extruder or a microfluidizer. Unencapsulated hemoglobin is separated by filtration, before PEGylation is performed by postinsertion. The resulting PEG-LEH is converted into oxyhemoglobin form and concentrated to obtain final product. Abbreviation IXC, interaction chamber.

High concentrations of H202 convert II into compound III, which is thought to be the same as the oxyperoxidase that is formed upon addition of 02 to the Fe(II) form of the free enzyme (Fig. 16-14, step g) and corresponds in structure to oxyhemoglobin.228... 

Sims and Horn (S13) did not consider that the addition of urea to the sodium benzoate solution was of any advantage and observed speedy coupling at pH 6.0-7.0 without it. The addition of nitrite to the diazo blank was found to eliminate errors due to hemolysis, and colorimetric readings were made at 525 mp since this is the isobestic wavelength for oxyhemoglobin and methemoglobin. Urea-ethanol solution has also been used to facilitate rapid coupling of bilirubin with diazotized sulfanilic acid. Patterson et al. (PI) have been able to eliminate the cloudiness that tends to develop with this method by the addition of concentrated phenol. 

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