Concentration of particular analytes

As with all techniques there may be limitations to the use of clean-up techniques. Sample loading may exceed the capacity of the clean-up column. Some of the interfering compounds may have similar structures and their behaviour with regard to polarity and sorbent phases may be similar, thus evading clean-up. Some analytes of interest may be lost due to poor technique or choice of solvent or sorbent. An understanding of the chemistry of the analytes of interest and interferences may overcome some of the limitations. 

Once the sample preparations are complete, a number of techniques are available to quantify petroleum hydrocarbons  

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With due regard to sampling, measurement and data analysis considerations, the application of IR spectroscopy to tissue, fluids and cells yields a remarkable amount of information. This information may relate to the concentration of particular analytes in biological fluids, the distribution of analytes within tissue, or the nature of biochemical changes associated with the disease process. In addition, information concerning metabolic processes in tissues and cells may be obtained. Examples will be presented to illustrate the general applicability of the technique. 

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