Concanavalin VOLUME

Fig. 7A—C. Biochemo-mechanical function of NIPA gel with immobilized concanavalin A (A) Schematic illustration of saccharide-responsive, reversible swelling of a NIPA gel loaded with concanavalin A. Na DS"- is dextran sulfate sodium (DSS). (B) Temperature dependence for equilibrated volume of NIPA gel including the Con A-DSS complex (DSS-gel, O), MP (MP-gel, ), and free of both DSS and MP (A). The latter was prepared as a control sample as described in the text except for the use of an aqueous Con A solution instead of the Con A-DSS solution. Hysteresis was observed in the volume changes of the MP-gel and free-Con A gel on heating and cooling, indicating a discontinuous phase transition. The diameter of each gel in the collapsed state, determined at 50 °C, was d0 = 0.074 mm the volume of this gel is denoted Vp. The concentration of dry matter in the collapsed state was estimated from the preparation recipe to be 90 wt%. (C) Repeated swelling/shrinking control at 34.5 °C by alternate binding of DSS and MP to gel-entrapped Con A. (E. Kokufuta, Y.-Q. Zhang and T. Tanaka [78])...

Four different amounts of the four gangliosides were added to microtiter wells containing human PBMC s and 18 micrograms of concanavalin-A. All four gangliosides markedly inhibited lymphoblastic transformation when 12.6 nanomoles was added to the cultures with a total volume of 0.2 ml (Table II). As little as 1.6-nanomoles of GDlb and GTlb resulted in substantial inhibition, while much less inhibition occurred with equivalent amounts of GM1 and GDla. 

Affinity Chromatography. The interaction of concanavalin A with alpha-D-glucans has been used in the determination of the fine structure of amylopectin and glycogen(88), where correlation between the external chain length of the glucan and the hydrodynamic volume has been established. 

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