Complementary separations, glycopeptide

Fig. 2-5. Examples showing the complementary separations on glycopeptide CSPs. (A) Separation of N-CBZ-norvaline on vancomycin (left) and teicoplanin (right). The mobile phase was methanol 1 % triethylammonium acetate (20/80 v/v) pH 4.1. (B) Separation of warfarin on teicoplanin (left) and vancomycin (right) CSPs. The mobile phase was acetonitrile 1 % triethylammonium acetate (10/90 v/v) pH 4.1. (C) Separation of naproxen on teicoplanin (left) and ristocetin A (right). The mobile phase was methanol 0.1 % triethylammonium acetate (30/70 v/v) pH 4.1. All columns were 250 x 4.6 mm i.d. The flow rate for all the separations was 1 mL min1 at ambient temperature (23 °C).

The macrocyclic glycopeptide chiral selectors are now a very important class of CSPs that must be part of the column set of any laboratory involved in enantiomeric separations. The variety of functionalities found in these relatively small molecules allow for many different interactions leading to successful enantioseparations [29]. The similarities between members of this class of chiral selectors produced the complementary separation property [14, 30, 31]. If a partial separation of an enantiomeric pair is observed on a macrocyclic selector, say vancomycin, a baseline separation may very likely be observed on a different selector, say teicoplanin. This interesting property in method development illustrates the large number of selector-selectand possible interactions. Such complementarities are due to the... 

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