Competitive Activity-Based Proteasome Profiling

Comparative and Competitive Activity-Based Proteasome Profiling 

1) In this chapter, cartoons of representative SDS-PAGE gels are shown. Original data can be found in referenced articles. 

Box 12.1 Tagging and Resolving by One- and Two-dimensional SDS-PAGE of Proteasomes by Activity-based Protein Profiling 

A protocol for proteasome labeling in vitro and in situ with broad-spectrum biotin-or Bodipy-containing peptide vinyl sulfones or peptide epoxyketones. 

In situ labeling Grow cells till mid log-phase. Incubate the cells with inhibitor 

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