Comets ethanol

It is essential that, with the exception of the ethanol wash, the solutions used in the protocol are agitated vigorously during use. Failure to do so will increase the risk of generating comet-tails and other undesirable patterns of background caused by localized reattenuation of excess cDNA material from the spots. 

Cairns and Neustadter (1975) studied the stability of carbon dispersions stabilized by BP 45, a copolymer of methacrylic esters for which R in the ester group -COOR could be the straight chain C4 or Cg-iz alkyl groups, or the amino substituted ethyl group -CH2CH2NH2. They also studied the stabilizer PV30-TEPA, a succinimide terminated poly(isobutylene). Flocculation was induced by the addition of ethanol to the dispersion medium, which was n-heptane. Their results, which compare the CFVs with the 0-compositions determined by the method of Comet and van Ballegooijen (1966), are presented in Table 9.1. 

Datta J, Dutta A, Mukherjee S (2011) The beneficial role of the Cometals Pd and Au in the carbon-supported PtPdAu catalyst toward promoting ethanol oxidation kinetics in alkaline fuel cells temperature effect and reaction mechanism. J Phys Chem C 115(31) 15324—15334... 

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