Combined Syntheses Employing Several Enzymes

Although it is not necessary to determine kinetic parameters in order to use enzymes for chemical synthesis, this knowledge can often be useful in dedding which avenues offer the best opportunities for process improvements. Likewise, it is not essential - but often highly useful - to understand the mechanism of the enzyme-catalyzed reaction for the same reasons that one often benefits from knowing the mechanisms of any reaction employed in a synthetic route. Although every individual enzymatic reaction has a uitique combination of kinetic properties and reaction mechanism, several useful generalizations are summarized in the subsequent section. 

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