Combinatorial library system

Drewry D H and S S Young 1999. Approaches to the Design of Combinatorial Libraries. Chemometrics in Intelligent Laboratory Systems 48 1-20. 

Another example for the use of hydrogen as reductant is observed in the reduction of imine [5b]. New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water-tetradecane solvent system. 

Gaecia-Egido, E., Spikmans, V., Wong, S. Y. E., Warrington, B. H., Synthesis and analysis of combinatorial libraries performed in an automated micro-reactor system. Lab. Chip 3 (2003) bl-TL. 

A combinatorial library of fluorescent NBD molecules was used to visualize subcellular transport pathways in living cells, using a kinetic, high content imaging system to monitor spatiotemporal variations of intracellular probe distribution <2004MI414>. 

The last work pertaining to the discovery of new catalysts is perhaps the most novel approach to be reported thus far. In one of the earliest approaches taken toward catalyst development, Menger et al. (61) attempted to find catalysts for phosphate ester hydrolysis. A series of eight functionalized carboxylic acids were attached to polyallylamine in various combinations. Each of these polymers were then treated with one of three metals, Mg2+, Zn2+, or Fe3+. The different members of each library were identified by the relative percentages of each carboxylic acid attached to the polyamine. For example, one polymer possessed 15% Oct, 15% Imi, 15% Phe, and 5% Fe3+. There is no attempt to identify the location of the various carboxylic acids in a given polymer. This approach is novel since each system consists of an ensemble of different ligands with the carboxylic acids positioned in various locations. Each polymer within a given ratio of carboxylic acids consists of a combinatorial library of potential catalysts. 

The discovery of DHFR inhibitor NGD-157 demonstrates that ALIS is an efficient system for identifying novel, bioactive lead compounds from large combinatorial libraries. A single ALIS experiment containing over 2500 compounds is complete in under 10 min, allowing more than 250 000 compounds to... 

A reversible Ugation technique was desired that would aUow the use of the mPE system in dynamic combinatorial libraries (DCL) in an effort to identify masterpiece sequences [81]. The imine bond metathesis is known to have an equilibrium constant close to unity, undergoes reactions at reasonable rates at room temperature, and has a geometry that is compatible with the phenylacetylene unit [82] therefore it was chosen as a component for mPEs. 

Corbett, P. T Sanders, J. K. M. Otto, S. Systems chemistry Pattern formation in random dynamic combinatorial libraries. Angew. Chem. Int. Ed. 2007, 46, 8858-8861. 

Bohl M, Dunbar JB, Gifford EM, Heritage T, Wild DJ, Willett P, Wilton DJ. (2002) Scaffold Searching Automated Identification of Similar Ring Systems for the Design of Combinatorial Libraries. Quant. Struct.-Act. Rel. 21 590-597. 

Yurek, D. A., Branch, D. L., and Kuo, M. S. (2002) Development of a system to evaluate compound identity, purity, and concentration in a single experiment and its application in quality assessment of combinatorial libraries and screening hits. 

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