Fingerprint column

Figure 4 Crossover of peak retention times as a function of gradient rate in the separation of peptides. (Previously unpublished data are drawn from Swadesh, J. K., Tryptic fingerprinting on a poly(styrene-divinyl benzene) reversed phase column, /. Chromatogr., 512, 3215, 1990.92)...

Swadesh, J. K., Tryptic fingerprinting on a poly(styrene-divinyl benzene) re-versed-phase column,. Chromatogr., 512, 315, 1990. 

Figure 6 High-speed tryptic fingerprint. Horse cytochrome c was digested with trypsin and the peptide chromatographed in acetonitrile water 0.1% TFA at various temperatures and flow rates on a 15 x 0.2-cm PS-DVB column packed with 3-p, 300-A particles, (a) 26°C and 0.5 ml/min. (b) 42°C and 0.7 ml/min. (c) 70°C and 1.1 ml/min. Detection at 220 nm. Note that the resolution rises with the speed of separation.89 (From Swadesh, ]., BioTechniques, 9, 626, 1990. With permission.)...

Different authors used RP-HPLC and UV detection to monitor peptide formation during cheese ripening [174-178], providing valuable information about proteolysis. When large hydrophobic peptide need to be separated an lEC represents the best choice [179]. Nevertheless, the identification of these peptides is essential for the complete understanding of the proteolytic process. The peptides eluted from the LC column can be subjected to ESl-MS for molecular weight determination and MS/MS for amino acid sequence determination, which allow rapid peptide identification [172]. HPLC-ESl-MS and MS/MS techniques have been successfully used for peptide mass fingerprint purposes for sequence analysis of purified albumin from Theobroma cacao seeds [180,181]. 

Table IV. Cyanogen Bromide Molecular Weight Fingerprint Data from 6% and 10% Agarose—GuHCl Columns...

The array has a length N for the total number of protein-ligand complexes and a width K of SIFt fingerprints bits. The value of each element of P(r) is derived by averaging the elements in each column of the SIFt matrix, yielding a... 

Petroleum fuel analysis does not require second column or second detector confirmation. Fuels are identified based on their fingerprints or characteristic patterns of multiple peaks similar to ones shown in Figure 2.5. Each peak represents an individual chemical constituent, and each fuel has a unique combination of these constituents forming a characteristic pattern or a fingerprint. The fingerprints obtained... 

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