Column evaluation asymmetry factor

The use of MIPs as chromatographic stationary phases is the most studied application of MIPs. This method is, in fact, the best way to quickly and efficiently validate the performance of a developed MIP. To achieve this, the MIP is packed into an HPLC column and the retention characteristics of the template and/or analogue molecules are collected in various selected mobile phases. From the collected data, useful parameters, such as capacity factor, imprinting factor, and peak asymmetry, are calculated and used to evaluate polymer affinity, cross reactivity, and other features of the MIP. 

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