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Column chromatography basic concepts

Like dissolves like is the basic concept for the selection of solvents in the eluent for liquid chromatography. Controlling the solubility of analytes is the key to success. If the selected solvent or mixture of solvents does not interfere with detection, it is a good eluent. The selection of a suitable solvent for low-wavelength absorption detection and post-column derivatization detection is important to obtain highly sensitive detection. The selection of a volatile solvent is the key for preparative-scale liquid chromatography and for mass spectro-metric detection. [Pg.89]

The usefulness of an analytical technique is directly related to an understanding of its theory. This part of the book will explain the basic concepts of gas chromatography (GC), the purpose and selection of columns, and the interpretation of the chromatographic record, that is, the chromatogram. The information in this part will give the reader sufficient information to understand what GC is, how the separation occurs, and how to relate data to sample composition. [Pg.38]


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See also in sourсe #XX -- [ Pg.141 , Pg.142 ]




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