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Size exclusion chromatography column broadening

The main disadvantage of size-exclusion chromatography is its low peak capacity, which arises from the small elution volumes of the peaks. In addition because peak dispersion is small, extra-column contributions to band broadening in size-exclusion chromatography have to be kept to a minimum. The relative contribution of extra-column band broadening can be minimized by the use of small particles = 3-10 pm) and long (L = 50 100 cm), wide-bore (d = 8-10 mm) columns. [Pg.75]


See other pages where Size exclusion chromatography column broadening is mentioned: [Pg.318]    [Pg.212]    [Pg.270]    [Pg.15]    [Pg.20]    [Pg.147]    [Pg.157]    [Pg.4926]    [Pg.140]    [Pg.96]    [Pg.387]    [Pg.90]    [Pg.103]    [Pg.189]    [Pg.455]    [Pg.414]    [Pg.405]   
See also in sourсe #XX -- [ Pg.20 ]




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