Column blank defined

This file introduces the concept of variables within the molecule specification. Here, variables are simply named constants variable names are substituted for literal values within the Z-matrix, and their values are defined in a separate section following it. The two sections are separated by a blank line, or a line with a blank in the first column and the label Variables placed elsewhere on it (this is one exception Gaussian makes in its requirement for completely blank lines). ... 

The increase or appearance of peaks has several possible explanations. Addition of contaminants by the base during extraction is minimal, as proven by GC analysis of blank ether extractions of the buffer used in the procedure. The base could convert compounds and thus account for some of the new peaks seen. However, the hump obscures the normal column base line, and the removal of the hump by base extraction may cause previously undetected or unintegrated compounds to be measured. The hump-associated retention times have more detector noise than the other retention times in the chromatograms. The integrator usually defines the detector noise as a peak unless the sensitivity of the integrator is decreased (22). 

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