Colony hybridisation

Figure 3.14 Hybridisation in direct cloning to identify wherein the heterologous DNA of interest can be found within a clonal library. DNA from each clone is isolated by treating each colony with alkali and drying. A labelled probe is then use to hybridise to the target in order to identify in which colony the desired gene/DNA of interest can be found (Reproduced from Voe, Voet Pratt, 1999 [Wiley] Fig. 3-31).

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