Colloidal gold immunogold

Fig 2 Immunogold negative staining, with a monoclonal antibody (JIM 5 (13)) that recognises a relatively unesterified pectic epitope, of rhamnogalacturonans extracted from onion cell walls. Arrows indicate 5 nm colloidal gold particles. Scale bar represents 200nm. 

Fig. 1. Diagram of an EM immunogold assay localizing a protein on plastic sections. The primary antibody binds to an exposed surface epitope of the embedded cells. The antibody is then visualized by binding a second antibody coupled to a colloidal gold particle. The electron-dense gold particle visibly marks the position of the bound antibodies when visualized with the electron microscope. 

Taban, C. H., and Cathieni, M. M. 1995. Microwave-aided binding of colloidal gold-protein-sub-stance P. In Immunogold-Silver Staining Principles, Methods, and Applications (M. A. Hayat, ed.), pp. 183-195. CRC Press, Boca Raton, FL. 

Key Words Colloidal gold labeling immunochemistry in situ hybridization immunogold nanogold undecagold cluster GOLDFISH. 

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