Collection, of yeast

National Collection of Yeasts Cultures, maintained of the Brewing Ind. Res. Foundation, Nat-field, Surrey, England... 

Candida boidinii (CCY 29-37-13) was obtained as an isolate from contaminated colunm of immobilized polygalacturonase, where 0.5% sodium pectate in 0.1 M acetate buffer, pH 4.6 was used as a substrate. Four other strains for comparison were obtained from Culture Collection of Yeasts, Institute of Chemistry (strains CCY 29-37-1, CCY 29-37-2, CCY 29-37-8, CCY 29-37-12). 

The cultivation of this yeast strain on pectin medium showed optimal grow conditions. The behaviour of this strain was compared with that of four strains of Candida boidinii from the Culture Collection of Yeasts. The grow curves of all strains on pectin medium showed marked plateau suggesting the presence of two existing C-sources in the pectin medium, requiring two different metabolic paths (Fig. 1). 

NC YC National Collection of Yeast Cultures, Brewing Industry Research Founidation,... 

National Collection of Yeast Cultures Maintained by the Brewing Ind. Res. Found, Nutfield. Surrey, U.K. NCYC... 

The following abbreviations are used ATP, ADP, and AMP for adenosine 5 -tri-, 5 -pyro-, and 5 -mono-phosphate UDP for uridine 5 -pyrophosphate NAD or NADH for nicotinamide adenine dinucleotide or its reduced form NADP or NADPH for nicotinamide adenine dinucleotide phosphate or its reduced form NCYC for National Collection of Yeast Cultures and CBS for Centraalbureau voor Schimmelcultures. 

Each species is made up of one or more strains. For example, by November, 1972, only one strain of Brettanomyces abstinens had been reported. By contrast, very many strains are known of the baking and brewing species, Saccharomyces cerevisiae the 1970 edition of the catalogue of the National Collection of Yeast Cultures (NCYC)86 lists 357 strains of this species. A strain is a culture and its subcultures, derived from a single isolated cell (cf, Refs. 87 and 88). 

The yeast strains used were D. hansenii CCMI941, B stipitis CBS 5773, and K. marxianus CBS 6556, obtained from the National Collection of Yeasts Cultures (UK), as NCYC 2597. The strains were maintained on YM agar slants containing 20 g 1 glucose, 3 g 1 yeast extract, 3 g l malt extract, 5 g 1 peptone, and 20 g F agar. 

CLIB Collection de Levures d lnt rSt Biotechnologique (collection of yeasts of Biotechnological Interest) INA-PG, Grignon, France. FCEB Faculte Olnologie de FUniversitS de Bordeaux II, Talence, France. 

ATCC = American Type Culture Collection, 2112 M-Street N.W., Washington D.C.7, U.S.. NCYC = National Collection of Yeast Cultures, Brewing Industry Research Foundati Nutfield, Surrey. 

S. pombe strains are available from public repositories, for instance the National Collection of Yeast Cultures (NCYC, www.ncyc.co.uk), or from the National BioResource Project (Yeast) (yeast.lab.nig.ac.jp/nig/index en.html). 

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