Collagen stock

Dilute collagen stock solution to 50 pg/mL in 0.02 N acetic acid. 

Gelatin microcarriers are manufactured from denatured porcine collagen (gelatin) obtained from processing pig skin from certified slaughterhouses and from stock certified to be free of transmittal disease. After denaturation under harsh thermal... 

The parameters required to create a 10-line exponential gradient of 0.2%, 1 nM NGF at the explant using incremental deposition of the volume of chemotropic factor onto the collagen gel, when the concentration of the stock remains constant in the microdispenser... 

Hide glues from hide trim and splits are prepared by initial washing with water. The stock is then soaked in lime (calcium hydroxide) and water for a period of weeks, which dissolves and removes extraneous protein-related materials, as well as conditioning the collagen for subsequent glue extraction by hydrolysis. The conditioned collagen is then washed with water, followed by addulation with dilute acid such as sulfuric, hydrochloric, or sulfurous, for pH adjustment, followed by a final water rinse. 

Votteler, M, Carvajal Berrio, D.A., Pudlas, M., Walles, H, Stock, U.A., and Schenke-Layland, K. (2012) Raman spectroscopy for the non-contact and non-destructive monitoring of collagen damage within tissues. J. Biophotonics, 5, 47-56. 

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