Cohn I fibrinogen

Figure 5 depicts similar results for Cohn I fibrinogen. Air drying was shown to have a marked disruptive effect on the adsorbate (Figure 5a). The morphology of the adsorbate differed from that of the air-dried y-globulin however, the morphology and pore size of the critical-point-dried adsorbate (Figure 5b) were similar to those for y-globulin. Comparison of the...

Figure 5. Cohn I fibrinogen, 3 mg/dL, adsorbed on Teflon (partial gold decoration technique). Key a, air dried, sintered Teflon b, critical-point dried, sintered Teflon and c, critical-point dried, Fluorofilm Teflon. Bar equals 0.1 p,mfor b and c.

Stereo pair TEM showed that the separation of albumin microadsorbates was preserved in surface holes and cracks, in contrast to the Cohn I fibrinogen results. 

Preexposure of the Flourofilm Teflon surface to albumin, followed by Cohn I fibrinogen (in an air-free, degassed solution environment) and... 

Figure 9. Albumin, 2500 mg/dL solution, preadsorped on sintered Teflon followed by Cohn I fibrinogen, 300 mg/dL, lh each (critical-point dry and PGDTEM techniques). Key bar equals 0.1 pm.

Figure 10. Summary of albumin preadsorption results on a number of polymers. Air drying and PGDTEM were used for all samples. Key F, 30 mg % fibrinogen (Cohn I) and A Fy 2500 mg % albumin followed by 30 mg % fibrinogen (Cohn I).

B igure 3. Cohn I human fibrinogen, 3 mg/dl, adsorption on Teflon gold decoration TEM technique. (a) air dried (b) critical-point dried with bar equal to 0.1 ym. (Reproduced with permission from Ref. 5. Copyright 1982 American Chemical Society.)... 

Figure 4. Cohn I human fibrinogen, 30 mg/dl, adsorption on Teflon partial gold decoration TEM technique. Bar equal to 1000 A.

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