Cofactor Recycle in Multi-Step Oxidizing Biocatalytic Systems

Cofactor Recycle in Multi-Step Oxidizing Biocatalytic Systems 

Technologies for recycle of cofactors in single-electron biocatalytic processes have been extensively studied and many reviews have been published (e.g. [50] and references therein). Clearly, if several redox enzymes are incorporated into 

New supramolecular compounds mimicking biological cofactors have been proposed which have high redox potentials and, further, can be Hnked covalently onto supports in biotransformation systems [52]. Bioelectrochemical methods available for regeneration of nicotinamide- and flavin-dependent systems are comprehensively reviewed by Kohhnan et al. [53]. 

Examples of Multi-Enzyme Biotransformation Processes Involving Oxidizing Enzymes 

A key consideration in development of all multi-step bioprocesses is the type of bioreactor it may be necessary to accommodate a range of conditions including compartmentalization of the enzymes, cofactor recycle, adequate oxygen supply, variable temperature and pH requirements, and differential substrate feed rates. Examples described below include a range of different reactors, of which membrane bioreactors are clearly often particularly useful. 

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