Cobyrinic acid-a,c-diamide

Cobalt insertion J, 6.6.1.2 Cobyrinic acid a,c-diamide cobNST ... 

Cobyrinic acid acts as the substrate for the first of two amidases, CbiA, which amidates the a and c acetic acid side chains (Figure 24). As with CobB, CbiA uses glutamine as its preferred amino donor and requires ATP as an energy source. Kinetic analysis has shown that the hydrolysis of glutamine and the synthesis of cobyrinic acid tf,c-diamide are uncoupled. It was also shown that the amino groups are transferred in the order of r first and then a, allowing a model to be proposed whereby phosphinylated intermediates are formed. ... 

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