Coating conjugates, preparation

Dilute anti-(mouse-lgG)-lgG (in the case of monoclonal antibodies for testing conjugates prepared from antibodies of other origin, use the respective anti-species specific antibody) to 5 pg/ml in Soln. A. Coat the wells of a microtest plate with 100 pl/well of this dilution and incubate at 4 °C overnight. Remove antibody solution and wash once with TBS. Add 150 pi Soln. C per well and incubate at... 

Beyond polyclonal antibodies, monoclonal antibodies to isoxazolyl penicillins were recently produced by immunization of mice with a cloxacillin-human serum albumin conjugate prepared by a mixed anhydride procedure (35). Sensitivity and specificity of these antibodies were tested in an indirect ELISA in which a cloxacillin-glucose oxidase conjugate prepared by an activated ester procedure served as a coating agent. It was found diat die prepared antibodies could be... 

In each of these methods, undiluted serum, urine, acid-deproteinized milk, or a buffered saline extract of muscle was mixed with sulfamethazine-horseradish peroxidase and added to antibody-coated wells of a microtiter plate. A sulfamethazine-bovine serum albumin conjugate prepared by the glutaraldehyde procedure (56) was used for antibody production. Results showed that screening of serum was of value since sulfamethazine concentrations in serum directly correlated with those in swine tissues. Thus, for example, a level of 100 ppb of sulfamethazine in... 

Fig. 4. Testing of a conjugate prepared from purified antibodies to mouse immunoglobulin labeled with alkaline phosphatase. Ordinate conjugate dilution abscissa absorbance at 405 nm of samples diluted 1 4 after 15 min of incubation with substrate. — , Dose response in microtiter wells coated with mouse serum proteins O—O, dose response in uncoated wells.

Nanoparticles of the conjugated polymers Me-LPPP and MEH-PPV were produced with the miniemulsion method. Monolayer of the particles on glass with ITO coating was prepared by spin-coating. The content of components in Me-LPPP MEH PPV blend is 2 1. 

Immunoassay. All samples were analyzed for trlazlne herbicides using RES-I-MUNE Immunoassay kits (ImmunoSystems Inc., Scarborough, Maine). The kits use polyclonal antibodies coated on the walls of polystyrene test tubes and an atrazine-enzyme conjugate prepared by covalently binding atrazine to horseradish peroxidase by a modified carbodilmide technique (12-13. Other reagents in the immunoassay kits include three standards (negative control, 0.1 ug/L atrazine solution and 1,0 ug/L atrazine solution), substrate, chromogen, and a "stop" solution of 2.5 N (normal) sulfuric acid. 

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