Cloning host choice

One way of alleviating a number of these problems is actually to make several parallel clone banks using different expression vectors and strains, regulatable expression systems, and low copy-number vectors. Vectors choices [28] such as plasmids vs phagemids, high vs low copy number vectors, all can affect what is cloned, and there is not one solution which will satisfy every project. Furthermore, the cloning host also makes a difference. E. coli is usually the host of choice, but Bacillus can be preferred for secretable enzymes, and yeast for eukaryotic or post-transitionally modified enzymes. 

The use of other hosts for HTP cloning, expression, and purification has been much more limited than E. coli, which remains the system of choice for high-level protein production. However, for many mammalian and viral proteins, problems of... 

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