CLEC columns

The preparative capacity of CLEC columns is reasonable. However, the presence of copper in the mobile phase is not convenient. Copper can be removed using a iminodiacetic acid-based resin column. ... 

The base matrices are divided into two groups. The inorganic matrices are based on silica or zirconia, on which the transition metal ion is complexed with the ligand bonded on the matrix. The inorganic sorbents have high column efficiency but low capacity and low chemical stability. The hgand can also be coated prior to, or during, the experiment, on the octadecylated silica (ODS) reversed phase, which presents the most inexpensive but efficient and selective sorbent for CLEC. 

Table 1 summarizes the LEC studies since 1990. As a stationary phases for CLEC, the weakness of resin microspheres is in their low column efficiencies. The study of polypyrrole-coated polystyrene microspheres presented an effort to overcome the weakness, where a second electrokinetic interaction was added in addition to the coordination interaction. A high protein adsorption is always needed as a stationary phase for IMAC. The study of PHEMA microspheres modified with dye plus metal ion was interesting, where the protein adsorption was greatly increased by metal complexion plus dye affinity. 

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