Cleavage of a disulfide

Another crosslinker, SAED (Chapter 5, Section 3.9), can be used in a similar fashion, but instead of transferring a radioactive label, it contains a fluorescent portion that is transferred to a binding molecule after cleavage. Similarly, sulfo-SBED routinely is used to study protein interaction. Cleavage of a disulfide bridge after capture of interacting proteins results in transfer of a biotin label to the unknown prey protein (Chapter 28, Section 3.1). The biotin modification then can be used to detect or isolate the unknown interactor for subsequent identification. 

ClNNAMOYL CHLORIDE, 77 Claisen reaction, 7, 32 Cleavage of a disulfide, 23 Clemmensen reduction, 57 Cocoanut oil, 69 Condensation... 

In the first step, thioredoxin reductase reduces a small redox protein, thioredoxin, via enzyme-bound FAD. This involves cleavage of a disulfide bond in thioredoxin. The resulting SH groups in turn reduce a catalytically active disulfide bond in nucleoside diphosphate reductase ( ribonucleotide reductase ). The free SH groups formed in this way are the actual electron donors for the reduction of ribonucleotide diphosphates. 

Disulfide exchange is a spontaneous reaction that occurs under neutral or basic conditions and involves cleavage of a disulfide bond and incorrect... 

The cleavage of a disulfide without the breaking of a carbon sulfur bond is possible over a palladium catalyst in aqueous acid at room temperature and atmospheric pressure. Good yields of the mercaptans have been obtained in this way 26" 178... 

Cleavage of a disulfide bond (cystine residue) by performic acid. 

Supported reagents were further applied to achieve post-cleavage purification of products obtained by solid-phase synthesis. This is illustrated by an example from Ellman s laboratory Scheme 1.6.22). The p-tum mimetic precursor 43 was released from solid support by TCEP (41) mediated cleavage of a disulfid linkage. The polymeric guanidine 44 was used to remove excess of 41 and of the phosphinoxide 42, to promote formation of the cyclic sulfide 45, and to trap the byproduct HBr from solution. 

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