CLEA/CLEC cross-linked enzyme

Figure 4 The different approaches to the production of carrier-free immobilized enzymes (A) crystallization (B) aggregation (C) spray-drying and (D) direct cross-linking. AGG, aggregates CLE, cross-linked dissolved enzyme CLEA, cross-linked enzyme aggregate CLEC, cross-linked enzyme crystal CSDE, cross-linked spray-dried enzyme CRY, crystals SDE, spray-dried enzyme.

Enzyme Crystals and Precipitates. Solid enzyme preparations can be cross-linked with a bifunctional reagent to yield cross-linked enzyme crystals (CLEG) (21), or cross-linked enzyme aggregates (CLEA) (22) that are insoluble in aqueous as well as nonaqueous media. This maintains their catalytic activity and increases their thermostability and resistance to proteases. The activity of the preparation remains high because of the high purity of the protein. CLECs are not commercially available anymore. CLEAs can be dried and suspended in any type of solvent (23). 

Cross-linked enzyme aggregate (CLEA) Cfoss-linked enzyme crystal (CLEC)... 

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