Clathrin-coated vesicles, purification

Xie, X.-S., Stone, D.K., and Racker, E., 1989, Purification of a vanadate-sensitive ATPase from clathrin-coated vesicles of bovine brain../. Biol. Chem., 264 1710-1714. 

The earliest electrophoretic techniques concentrated mainly on the preparation of mitochondria " and lysosomes, but further research has made possible the purification of secretory vesicles clathrin-coated vesicles " endoplasmic reticulum early, middle-, and late-stage endosomes peroxisomes " microsomes " and phagosomes." These preparatory techniques have functioned as the proof-of-principle for analytical separations of intact organelles using CE, by demonstrating that isolated organelles are amenable to eleetrophoretic separation techniques. 

Serafini, T., and Rothman, J. E. (1992). Purification of Golgi cisternae-derived non-clathrin-coated vesicles. Methods Enzymol. 219, 286-299. 

COPI-coated vesicles mediate intra-Golgi transport and Golgi to ER retrograde transport. The coats of these vesicles do not show the geometric forms seen with clathrin coats and have a more complex protein composition [3]. Coat protein purification first lead to the identification of a complex composed of seven individual coat-protein subunits, known as COPI or coatomer. Some of these subunits bear a sequence similarity to clathrin adaptors. In addition, there is a small GTP-binding protein, Arfl, present on COPI-coated vesicles. 

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