Classes denatured state

DSC of soy isolates show two distinct and different classes of behaviom. Where proteins are in the native state, distinct denatmation transitions from the 7S and 1 IS globulins are observed. For raw materials that have been denatured during their preparation by moist heat treatment or exposure to denaturing solvent conditions, no large endotherms associated with the native proteins are seen. (Morales and Kokini 1997) Glass transitions in soya proteins have been reported previously (Morales and Kokini 1997 Mizuno et al. 2000). 

For soluble and immobilized bromelain, temperature increase is accompanied by a decrease in residual enzyme activity. A more complex form of denaturation occurs with the immobilized enzyme, which may involve a two-phase process. Immobilization offers more resistance to denaturation at the higher temperature of 60°C where the second phase is prolonged by a factor of three [60]. Differential scanning calorimetry experiments showed that bromelain is an exceptional protease among the cysteine proteases, illustrated by the fact that its thermal denaturation is consistent with an irreversible two-state model [61]. Also, the far UV circular dichroism spectrum of bromelain differs from those of papain and chymopapain and therefore represents a third spectral class within the cysteine proteinase family [62],... 

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