Circular DNA, vectors

Fig, 5. Amplified production of silicatein protein from recombinant DNA cloned in bacteria. DNA coding for the silicatein a protein was integrated enzymically into a double-stranded circular DNA vector molecule to produce a recombinant DNA. When introduced into bacteria, the recombinant DNA is replicated by intracellular enzymes to produce many copies in each cell. These DNA molecules serve as templates, directing the synthesis of the silicatein protein they encode. Rapid reproduction of the bacteria greatly amplifies this synthesis. 

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