Circular dichroism recording spectrometer

Absorbance measurements were made by using a Cary IE UV-visible spectrometer (Varian, France), while circular dichroism spectra were recorded with a Jobin-Yvon Mark V dichrograph, both at 20°C, after dilution in water. 

Measurements. The absorption, circular dichroism (CD), and ESR spectra were recorded with a Hitachi 323 spectrophotometer, a JASCO MOE-1 magnetic circular dichroism spectrometer, and a JEOL JES-FE3X ESR spectrometer, respectively. The measurements of the spectra were carried out at 15°-20°C except for the ESR spectra, which were recorded at 77 K. The resonance Raman spectra were recorded at 10°C with 488.0-nm excitation (Ar+ laser) on a JEOL JRS-400D-002 spectrophotometer. The concentration of copper was determined to be 4.9mM by atomic absorption measured by a Nippon Jarrell-Ash AA-1 spectrometer. 

Apparatus. The spectrum and intensity of fluorescence were measured with a RF-540 spectrofluorometer (Shimadzu, Japan). An UV-260 (Shimadzu, Japan) spectrophotometer was employed in all absorption spectra recordings. The circular dichroism spectrometer JASCO J-810 (Hitachi. Japan) was used for measurement... 

Circular dichroism (CD) spectra were recorded on the Jasco model J-815 CD spectrometer, using measurement range at 190-250 nm and sample concentration of 0.8 mg/ml (SOOppm). The ratio of peak height to trough depth was calculated using below given Eq., described by Morris, et al, 1980 [64]. 

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