Circular dichroism and fluorescence

The significance of light absorption in biochemical studies lies in the great sensitivity of electronic energy levels of molecules to their immediate environment and to the fact that spectrophotometers are precise and sensitive. The related measurements of circular dichroism and fluorescence also have widespread utility for study of proteins, nucleic acids, coenzymes, and many other biochemical substances that contain intensely absorbing groups or chromophores.58... 

The study by Determan et al. [224] focuses on the effects of polymer degradation products on the primary, secondary, and tertiary structure of TT, OVA, and lysozyme after incubation for 0 or 20 days in the presence of ester (lactic acid and glycolic acid) and anhydride [sebacic acid and l,6-bis(p-carboxyphenoxy)hexane] monomers. The structure and antigenicity or enzymatic activity of each protein in the presence of each monomer was quantified. SDS-PAGE, circular dichroism, and fluorescence spectroscopy were used to assess/evaluate the primary, secondary, and tertiary structures of the proteins, respectively. ELISA was used to measure changes in the antigenicity of TT and OVA and a fluorescence-based assay was used to determine the enzymatic activity of lysozyme. TT toxoid was found to be the most stable in the presence of anhydride monomers, while OVA was most stable in the... 

Lin SS, Gether U, Kobilka BK. Ligand stabilization of the p2 adrenergic receptor Effect of DTT on receptor conformation monitored by circular dichroism and fluorescence spectroscopy. Biochemistry 1996 35 14,445-14,451. 

T. Osterlund, D.J. Beussman, K. Jule-nius, P.H. Poon, and S. Linse, Domain identification of hormone-sensitive lipase by circular dichroism and fluorescence spectroscopy, limited proteolysis, and mass spectroscopy, J. Biol. Chem., 1999, 274, 15382-15388. 

Kuramoto N., Sakamoto M., Komiyama J., Iijima T. Complex formation between bovine serum albumin and poly(acrylic acid) as studied by viscometry, circular dichroism, and fluorescence. Makromol. Chem. 1984 185 1419— 1427. 

Pasta P, Riva S, Carrea G (1988). Circular dichroism and fluorescence of polyethylene glycol-subtilisin in organic solvents. FEBS Lett. 236 329-332. 

Elove, G.A., Chaffotte, A.F, Roder, H., Goldberg, M.E. Early steps in cytochrome-c folding probed by time-resolved circular-dichroism and fluorescence spectroscopy. Biochemistry 1992,31,6876-83. 

M. Fujiki, H. Nakashima, J. R. Koe and H. Takigawa. UV-visible, circular dichroism and fluorescence spectra of polythiophenes with (5 )-2-methyloctyl side-chains. Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.) 40( 1), 523-524 (1999). 

Bertazzon, A. Tian, G.H. Lamblin, A. Tsong, T.Y Enthalpic and entropic contributions to actin stability Calorimetry, circular dichroism, and fluorescence study and effects of calcium. Biochem. 1990, 29, 291—298. 

Early Steps in Cytochrome-c Folding Probed by Time-Resolved Circular-Dichroism and Fluorescence Spectroscopy. 

Comparative circular dichroism and fluorescence studies of oligode-oxyribonucleotide and oligodeoxyribonucleoside methylphosphonate strands in duplex and triplex formation. Biochemistry 30, 1650-1655... 

In this work, the effects of the presence of anionic surfactants SDS and SDBS on the viscosity properties of the coagulant protein from Moringa oleifera are reported. The results of solution properties of the protein in the presence of SDS are verified by surface tension, circular dichroism, and fluorescence measurements reported earlier [15-18]. 

Singh, B. R. and DasGupta, B. R., 1989a, Structure of heavy and light chain subunits of type A botulinum toxin analyzed by circular dichroism and fluorescence measurements. Mol. Cell. Biochem. 85 67-73. 

Singh, B.R., Evenson, M.L. and Bergdoll, M.S. 1988a. Structural analysis of staphylococcal enterotoxins B and Cl using circular dichroism and fluorescence spectroscopy. Biochemistry 27 8735-8741. 

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