Conformation, circular dichroism and

Woody, R.W. (1996) Circular dichroism and conformational analysis of biomolecules (ed G.D. Fasman), Plenum Press, New York, pp. 25-27. 

S S CONTENTS Preface, C. Allen Bush. Methods in Macromo-lecular Crystallography, Andrew J. Howard and Thomas L. Poulos. Circular Dichroism and Conformation of Unordered Polypeptides, Robert W. Woody. Luminescence Studies with Horse Liver Dehydrogenase Information on the Structure, Dynamics, Transitions and Interactions of this Enzyme, Maurice R. Eftink. Surface-Enhanced Resonance Raman Scattering (SERRS) Spectroscopy A Probe of Biomolecular Structure and Bonding at Surfaces, Therese M. Cotton, Jae-Ho Kim and Randall E. Holt. Three-Dimensional Conformations of Complex Carbohydrates, C. Allen Bush and Perse-veranda Cagas. Index. 

Hashizume H, Imahori K (1967) Circular dichroism and conformation of natural and synthetic polynucleotides. J Biochem (Tokyo) 61 738-749... 

Kypr J, Kejnovska I, Renciuk D, Vorlickova M (2009) Circular dichroism and conformational polymorphism of DNA. Nucleic Acids Res 37 1713-1725... 

CIRCULAR DICHROISM AND CONFORMATION IN COPOLYMERS WITH AROMATIC SIDE CHAINS AND IN LOW MOLECULAR WEIGHT MODELS... 

Fasman G.D. (Ed.) Circular Dichroism and the Conformational Analysis of Biomolecules, Plenum Press New York, 1996. 

Using circular dichroism and nuclear magnetic resonance spectrometry, Veatch and coworkers1 established that four conformational species of gramicidin A exist in solution. Two were postulated to be helixes of opposite handedness. 

While it is tempting to explain regulatory and cosolvent effects on the basis of conformational changes favorable or unfavorable to enzyme activity, it is much more difficult to demonstrate the actual involvement, amount, and structural details of such changes. Experimental evidence consists in most cases of bits and pieces provided by techniques such as absorption and fluorescence spectroscopy, circular dichroism, and magnetic circular dichroism. These tools work in solution (and, when desired, at subzero temperatures) to investigate not simply empty enzymes but enzyme—substrate intermediates. However, even with this information, the conformational basis of enzyme activity remains more postulated than demonstrated at the ball and stick level, and in spite of data about the number and sequence of intermediates, definition of their approximate nature, rate constants, and identification of the types of catalysis involved, full explanation of any particular reaction cannot be given and rests on speculative hypothesis. 

M Kats, PC Richberg, DE Hughes. Conformational diversity and conformational transitions of a monoclonal antibody monitored by circular dichroism and capillary electrophoresis. Anal. Chem. 67 2943-2948 (1995). 

In conclusion, given a protein in which tryptophan is partially or wholly buried, quantitative estimates of quenching by different quenchers provide a further fingerprint that is specific to the particular conformation of a recombinant protein and which—combined with other fluorescence, circular dichroism, and gel electrophoresis data—forms the basis for specific identification and quality assessment. 

Circular Dichroism and Saccharide-Induced Conformational Transitions of Soybean Agglutinin... 

Firstly, conformational changes and/or the new formation of bonds in the complex (including rearrangements of bonds) were observed by means of circular dichroism and potentiometry as shown in Fig. 46. In the PGA-QOEI system, the a-helix of PGA is destabilized with aging. From the change in pH of the complex solution, it is found that even after the complex has been formed the further bindings are newly formed. In Fig. 46, ApH is the change of proton concentration calculated from the equation... 

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