Cinnamyl anthranilate conditions

In the second mouse lung adenoma assay, cinnamyl anthranilate was administered to 15 females and 15 males under similar experimental conditions, except that redistilled tricaprylin was used as the vehicle. The control group consisted of 80 females and 80 males. The numbers of tumours per mouse were increased at the high dose, being 0.85 0.23 (p < 0.01) in high-dose females, 1.40 0.36 p < 0.001) in high-dose males, 0.54 0.15 (p < 0.05) in low-dose females and 0.47 0.12 in low-dose males compared with 0.20 0.02 in control females and 0.24 0.03 in control males (Stoner etal., 1973). 

Chronic administration of peroxisome proliferators to rodents results in sustained oxidative stress due to overproduction of peroxisomal hydrogen peroxide. The induction of peroxisomal fatty acid P-oxidation by cinnamyl anthranilate in vivo under bioassay conditions (Lake et al, 1997) supports this h othesis. Other data on the induction of oxidative stress are not available for cinnamyl anthranilate. 

Similarly, the modulation of hepatocellular proliferation by peroxisome proliferators has been implicated in the mechanism of carcinogenesis. This can theoretically result in increased levels of mutation by increasing the frequency of replicative DNA synthesis as well as increasing the number of hepatocytes at risk. Furthermore, hepatocellular proliferation is likely to be involved in the promotion of growth of preneoplastic hepatocytes. There is clear evidence that cinnamyl anthranilate causes acute and sustained levels of hepatocellular proliferation under bioassay conditions which resulted in liver tumours in mice. Interestingly, the magnitude and duration of hepatocellular proliferation were limited in rats, which did not respond with liver tumours in the bioassay (Lake et al., 1997). 

Under conditions of the bioassays, cinnamyl anthranilate induced peroxisome proliferation and cell replication in the liver that are characteristic of a peroxisome proliferator in mice and, to a limited extent, in rats. 

Table I. Effect of Diffusion Cell Conditions on the Absorption of Cinnamyl Anthranilate (I) (Cortisone Control) ...

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