CIDNP analysis of glycoproteins

When CIDNP-reactive amino acids are accessible on the surface of a glycoprotein, a possible impact of the covalently bound oligosaccharide chain on the protein part can be scrutinized [14, 15, 61] by this method. Therefore, CIDNP-experiments are also suitable to improve structural models of glycoproteins. On the basis of a X-ray structure of a glycoprotein, very often only the coordinates for the protein part are available since the flexibility of the oligosaccharide chain hampers a complete crys- 

In summary, the CIDNP method is a potent addition to the arsenal of biophysical methods which are successfully used in the field of structural glycosciences. The combination of X-ray data, molecular modelling, trNOE-methods and laser-photo CIDNP provides valuable structural information about protein-carbohydrate complexes. This is of particular importance, when multidimensional NMR-data can not be recorded due to the size of the molecule. Furthermore, the analysis of similar carbohydrate-binding proteins sharing a sufficient sequence homology is possible when complete structural data are available only for a few of them and CIDNP-and molecular modelling data are present for all of them. The successful role, which CIDNP-experiments can play for the solution of important structural glyco- 

This work was supported by the Human Capital and Mobility Program of the European Community, the Netherlands Foundation for Chemical Research (SON) and the Netherlands Organisation for Scientific Research (NWO). 

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