Chromatography UV spectroscopy

For small molecule analytes (see Note 6) for which a radiotracer form is available, sequentially load a known quantity of tracer dissolved in buffer and determine the amount of analyte in the eluant. When the radioactivity not retained by the immunoaffinity column plateaus, the column binding sites are saturated. Wash the column, and elute the retained radioactivity. The mass of analyte in the eluted volume is the apparent column capacity. In many instances a radio-labeled analyte may not be available. In such cases, high-performance liquid chromatography, UV spectroscopy, or any other analytical tool capable of selectively quantifying the analyte may be used to determine column capacity. 

Uses Solvent in petrol, refining dielectric fluid chem. intermediate raw material for drug mfg. high performance liq. chromatography UV spectroscopy... 

Table 3.1 Analysis of some standard polyethylene samples by combined column chromatography/UV spectroscopy ...

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